ABSTRACT
Objective To investigate the influence of electroacupuncture on the expression of neuropeptides in fibroeartilage callus tissue after femoral fracture. Methods Forty male Wistar rats were randomly divided into a fracture group and an electroacupuncture group. Femoral fracture models were established in both groups, and the rats in the electroacupuncture group were given electroacupuncture. Rats were sacrificed on the 4th, 7th, 14th and 28th days after surgery. The calluses were stained immunohistochemically to detect the expression of calcitonin gene-related peptide (CGRP) and substance P (SP). Results CGRP and SP levels increased rapidly after eleetroacu-puncture, with neuropeptides expressed strongly in callus cell tissue. The optical density (OD) in the electroacu-puncture group was significantly higher than that in the fracture group ( P≤0. 05 ). Conclusion Electroaeupune-ture can significantly increase the expression of neuropeptides in rats after femoral fracture. Electroaeupuneture can promote fracture healing through regulating neuropeptides.
ABSTRACT
[Objective]To explore the effect of spinal cord injury(SCI) repair by peripheral nerve combined with activitied macrophages graft.[Method]Fistly the preparation of the activated macrophages and fabrication of the peripheral nerve were done.81 BALB/C mice were grouped in A,B,C,D groups randomly after their spinal cord were hemi-resected.20 mice of group A were dealed with activated macrophages graft;20 of group B were dealed with peripheral nerve graft;21 of group C were transplanted both two graftes;20 of group D were contral group.Each of the 4 groups was respectively divided into 3 subgroups randomly.7 of subgroup 1 were investigated by their restoration of the motor function and improvement of the somatosensory evoked potential(SEP).7 of subgroup 2 were tabken for histological examination.The spinal cords were fixed and cut into longitudinal frozen sections,and were immuneostained and stained with hematoxylin and eosin(HE).The numbers of cells or neurofilaments were counted under microscope.6 of subgroup 3(7 of C3)were used as complement ones.All the results were recorded from 1 to 12 weeks postoperative and analyzed statistically using the SPSS 12.0 software package.[Result]The BBB scale and the SEP wave:there was a significant difference between group C1 and the other three groups,there was a significant difference between group A1,B1 and D1,too(ONE WAY ANOVA,P